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HotStart 2xTaq DNA Pol Master Mix,1ML, P03-CR03SM-1ML

Contains 1 ml of 2xMasterMix, 25 mM MgCl2, nuclease free water and DMSO. 2XMasterMix is premixed with sample loading dye for immediate loading after the PCR reaction..
HotStart PCR for specific amplification. Comparable with the Platinum Taq polymerase. Good for TA cloning..
Dye Minus HotSatrt 2X Master Mix is also available upon request..
Applications PCR amplification of DNA fragments up to 10 kb DNA labeling and DNA sequencing PCR for cloning. Quality Control No contaminating endonuclease or exonuclease activity detected. Functionally tested in PCR.

Isolate genomic DNA from both Gram-positive & Gram-negative bacteria.
High yield, high quality DNA for sensitive downstream applications including sequencing, PCR & qPCR.
Spin columns-collection tubes- 2 mL (50/bag).

Storage Buffer 20mM Tris-HCl, 1mM DTT, 0.1mM EDTA,100mM KCl 50% glycerol, 0.5%NP40, 0.5%TW20.
10×PCR Buffer 750 mM Tris-HCl (pH 8.8 at 25°C), 200 mM KCl, 50 mM (NH4)2SO4, 15mM MgCl2, 0.5% NP-40.
Unit definition One unit is the amount of enzyme required to catalyze the incorporation of 10 nmol of nucleotides into acid insoluble material in 30 min. at 74°C under assay conditions..
Applications PCR amplification of DNA fragments up to 5 kb DNA labeling and DNA sequencing PCR for cloning. Quality Control No contaminating endonuclease or exonuclease activity detected. Functionally tested in PCR.

Kit contains (4) DF columns, (4) 2mL collection tubes, 3mL DF buffer, 1mL wash buffer (4mL add ethanol) and 1mL elution buffer.
Sample: up to 300mg agarose gel slice and up to 100 microliter PCR product or other enzymatic reaction.
expectant yield is 80-90 percent for gel extraction and 90-95 percent for PCR clean-up.
Applications: PCR fluorescent or radioactive sequencing, restriction digestion, DNA labeling, ligation.

Fastest: 8 minutes from culture flask to high-quality plasmid DNA.
Pellet-Free: Direct lysis procedure omits cell-pelleting and resuspension steps.
High Quality: Ready for PCR, sequencing, cloning, and transfection.

Designed for use on the majority of PCR, qPCR and sequencing instruments with 384 well block.
Compatible with robotic systems and standard multichannel pipettes.
fit for use with ABI/LifeTechnologies qPCR instruments.
Frame: PC, rigid for added mechanical stability & improved seal integrity.
Wells: PP, thin-walled for optimum PCR/qPCR results.
003c30 µl working volume, 55 µl total well capacity.
Free from DNase, RNase, human genomic DNA, and endotoxin/pyrogen.

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PROBIOTIC WITH ENZYMES: Contains four key digestive enzymes that may assist the body’s natural ability to break down proteins, fats, carbohydrates....
TRUSTED PROBIOTIC SUPPLEMENT: No Refrigeration Required. Non-GMO, Gluten Free, Dairy Free. DNA Sequencing Verified for each strain. Stomach....
BALANCED DIGESTIVE SUPPORT: This probiotic is specifically formulated to maintain good colon health, relieve occasional digestive discomfort, promote....
THE FRIENDLY TRIO BACTERIA: Our proprietary blend of three clinically studied strains, L. gaseri, B. bifidum and B. longum, that have been shown to be....
EXPLORE WAKUNAGA: With a mission to promote good health throughout the world, Wakunaga offers nutritional supplements including Kyolic Aged Garlic....